A Method of producing Chromatin Staining in Sections

Ruthenium Red Staining of Chromatin in Epon Sections

For a long time, the ammoniated ruthenium oxychloride “ruthenium red” (today formulated [1, 2] as [(NH3)5 R u •O • Ru (NH3)4 O • Ru (NH3)5]6+ • Clg), has been used as a microscopic test for pectins [2, 3]. Several applications of this contrasting agent for electron microscopic detection of acid polysaccharides and proteoglycans have been stud­ ied [4-10]. Its use as a Schiff-like reagent was re...

Halomax® as a method for chromatin staining in cat sperm

A Routine Method for Obtaining High Contrast without Staining Sections

Immersion of tissue in aqueous uranyl acetate be-for giving some degree of contrast to components fore embedding is commonly practiced to increase such as membranes when they extend through the the contrast on sections which are subsequently thickness of the section parallel to the electron stained (2, 3, 5). The treatment is only satisfactory beam. A simple modification of current practice 540

A Method of Staining Thin Sections with Lead Hydroxide for Precipitate-free Sections

Staining of thin sections results in greater contrast which facilitates focusing and the observation of fine structure. Lead hydroxide, as suggested by Watson (1), has become increasingly popular as a stain for electron microscopy. However, when lead hydroxide is exposed to the carbon dioxide of air, lead carbonate forms and is deposited on the sections as fine, needle-like crystals, large amor...

Comparison of Three Staining Methods of Vibratome and Paraffin Sections in Preserving Thickness of Rat Hippocampal Sections

Purpose: To determine the optimal technique for maintaining hippocampus vibratome sections volume. Materials and Methods: This experimental study was performed on three rat hippocampus. The first hippocampus was embedded in paraffin and using rotary microtome forty sections were cut in a nominal thickness of 100 micrometer. They were then ordinary stained. The two others were cut into 80 sectio...